Digital Cameras Microscopy
biology lab question dealing with fluorescent microscopy?
1.Despite the fact that fluorescein fluoresces green, the digital CCD cameras used for capturing fluorescent microscopy images are all grayscale cameras (i.e., black and white). Why is it OK to use a monochrome digital camera to capture fluorescent images?
It all has to do with the filter. Flourescein is hard to type, so I’m going to say GFP for the rest of this answer…they aren’t the same, but for our purposes they are similar.
So in an epifluorescent scope, when the mercury lamp shines on the first filter, it allows only light around 475nm to pass through. When this light hits a GFP molecule, the molecule absorbs that light and emits light at around 509nm. This light passes though another filter that only lets light near that wavelength through. The camera on the top of the scope doesn’t see color, it sees only dark and light…but since the filter only lets on color through, all of the light the camera detects is from GFP fluorescences. When you change the filter to the other channels, the filters change so that the camera, again, only sees the light emitted by the fluorescent molecules.
Carson Z-pix Digital Zoom Microscope
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Celestron 44410 Prepared Microscope Slides (25-Piece Set) $14.88 You can sort slides from all sectors of the scientific world with the Celestron® 25 Assorted Prepared Slides. The slides cover insect parts, plant parts, and animal parts and come in a wooden case…. |
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Celestron 44340 LCD Digital LDM Biological Microscope $159.95 Celestron 400X LCD Digital Microscope with viewing screen and built – in digital camera. Give curious eyes the tools to explore their world! This amazing Microscope is ready to send a young mind into the realms beyond visible sight. Small mysteries are magnified up to 400 times to reveal the up-close details like never before… and it’s all shown in perfect clarity on the 3.5″ LCD screen! Even be… |
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